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We
have developed a proprietary protocol that can generate custom hybridomas
in two months or less. Quality is superior, unlike existing high
speed methods on the market. We accomplish this by using the latest
technology, automation, and proprietary processes developed by our
team. |
Proprietary
mouse generates antibodies to more epitopes on your immunogen. State of the art liquid handling makes it faster than traditional methods for developing hybridomas |
Three
mice will be immunized. Your immunogen will be emulsified with Freund’s
Complete Adjuvant. Booster injections are emulsified with Freund’s
Incomplete Adjuvant. After the last immunization, the antibody titers
will be assessed by ELISA. Immunogen requirements: 1-2 mg suitable for immunizations and ELISA plate coating. 0.5 mg negative control protein. |
Cells
from immunized mice will be prepared and fused with myeloma cells.
Viable hybridomas will be HAT selected and screened for antigen-specific
antibodies by ELISA. Up to 48 antibody secreting hybridomas with the best characteristics will be grown up and re-tested. Supernatants from positive hybridomas will be shipped to you for evaluation. |
Up
to five (5) positive parental hybridomas will be subcloned. One
(1) vial of each parental hybridoma will be frozen down with about
2x106 cells/vial for long term storage. Single colony wells will
be screened for antibody secretion by ELISA. Two (2) subclones from each parental hybridoma will be expanded and frozen down into three (3) vials each. |
| All subclones frozen down
in two (2) vials. All subclones (1 vial each) stored in our cell bank (6 months without additional charge). Supernatant from all subclones. Isotype of each parental line. |
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