High Throughput Protocol

Monoclonal Antibodies Delivered in only 40-60 days

Advantages of the HTP^TM Platform

• High speed
• High affinity
• Broad epitope diversity
• Ability to make antibodies to traditionally difficult targets
• Guaranteed results

Targets our HTP^TM Protocol has been shown to deliver superior antibodies against:

• Anti-idiotype targets
• Peptides
• Proteins
• High homology targets (human, rat and even mouse proteins)
• GPCRs
• 1-Amino acid specificity
• Small molecules
• Conformation specific

Technical Features

• Our proprietary HTP^TM mouse has been engineered with a boosted immune system to generate high affinity antibodies to more epitopes on your immunogen than previously existing mice. For more information, see our technology page.
• State of the art liquid handling makes it faster than traditional methods for developing hybridomas
• Experienced staff of scientists and customer service representatives use their expertise to help you design every project to optimize success
• Over 90% success rate

How the HTP^TM process works:

Phase I: Immunization (Day 1)

• Three HTP^TM mice are immunized. Your immunogen is emulsified with Freund’s Complete Adjuvant. Booster injections are emulsified with Freund’s Incomplete Adjuvant. After the last immunization, the antibody titers are assessed by ELISA.
• Immunogen requirements: 1.5 to 3 mg suitable for immunizations and ELISA plate coating. 0.5 mg negative control protein.  Let us know if this quantity is difficult to obtain; we can work with smaller amounts of antigen.  If the protein is not available, we can design and synthesize peptides for immunization and screening.

Phase II: Fusion (Day 21)

• Cells from immunized mice are prepared and fused with myeloma cells. Viable hybridomas will be HAT selected and screened for antigen-specific antibodies by ELISA.

Phase III: Screening (Finished by Day 35-45)

• Screening for most targets is done via ELISA.  We also have the capability to screen using flow cytometry, western blotting, affinity ranking, and others upon request.
• Up to 48 antibody secreting hybridomas per fusion with the best characteristics will be grown up and re-tested. 
• Optional: Supernatants from positive hybridomas can be shipped to you for evaluation.  This step usually takes place 40 days after immunization.
  • Note: If the customer decides to screen the supernatant samples, we ask that you are prepared ahead of time to receive and assay them so that we may move forward in a timely manner.  

Phase IV: Subcloning (Day 45-54)

• Up to five (5) positive parental hybridomas are subcloned. One (1) vial of each parental hybridoma is frozen down with about 2x10⁶ cells/vial for long term storage. Single colony wells are screened for antibody secretion by ELISA.
• Two (2) subclones from each parental hybridoma are expanded and frozen down into three (3) vials each.

Deliverables

Day 40:

• Up to 48 supernatant samples for our customer to screen for selection of hybridomas with desired characteristics for subcloning and delivery

Day 60:

• Up to 10 hybridoma subclones from 5 parentals specific to antigen by ELISA are cryopreserved in two vials per subclone.
• Supernatant samples from each subclone
• Isotype of each subclone 
• All subclones and parentals (1 vial each) are stored in our cell bank for 6 months without additional charge.
• Report detailing the work performed by Abpro

Other Services

Guarantee

Confidentiality